| 產品名稱 |
pUC-h27OH |
| 商品貨號 |
B177065 |
| Designations |
pUC-h27OH |
| GenBank Number |
M62401
|
| Species |
Homo sapiens, human |
| Depositors |
DW Russell |
| Applications |
contains sequence cytochrome P-450, sterol 27-hydroxylase cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypep, sterol 27-hydroxylase |
| Vector |
Construct size (kb): 4.579999923706055 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.884999990463257 Tissue: human prostate Gene product: cytochrome P-450, sterol 27-hydroxylase( cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypep, sterol 27-hydroxylase) [h27OH] Target Gene: cytochrome P-450, sterol 27-hydroxylase |
| Insert Size (kb) |
1.885 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; HindIII--3.0, 1.6; XbaI--4.6; BamHI--3.0, 1.6; PstI--2.9, 0.8, 0.7, 0.2. DNA sequence analysis of hybridization-positive clones predicted a human sterol 27-hydroxylase consisting of a 33-amino-acid mitochondrial signal sequence followed by a mature protein of 498 amino acids. |
| References |
Cali JJ, Russell DW. Characterization of human sterol 27-hydroxylase. A mitochondrial cttochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis. J. Biol. Chem. 266: 7774-7778, 1991. PubMed: 1708392
|
