| 產品名稱 |
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1] |
| 商品貨號 |
B177117 |
| Designations |
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1] |
| GenBank Definition |
Yeast (K.lactis) beta-galactosidase gene, 5' end |
| GenBank Number |
M18108
|
| Species |
Kluyveromyces lactis (Dombrowski) van der Walt |
| Depositors |
University of Kentucky, RC Dickson, University of Kentucky |
| Applications |
produces protein beta-galactosidase beta-D-galactosidase produces protein lactose permease This is a Lac+ strain expressing K. lactis lactose permease and beta-galactosidase. |
| Vector |
Construct size (kb): 20.0 |
| Insert |
DNA: genomic Insert lengths(kb): 12.0 Gene product: beta-galactosidase(beta-D-galactosidase) [LAC4] Target Gene: lactose permease, beta-galactosidase |
| Insert Size (kb) |
12.0 |
| Genotype |
MATalpha trp1-289 ura3-52 gal2 |
| Media |
ATCC® Medium 1245: YEPD
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
The plasmid contains the following restriction sites (approximate kb from the pBR322-derived EcoRI site): XhoI--2.5; EcoRI--8.1, 9.1, 11.4, 11.9, 12.6, 15.1, 16; BamHI--14.6, 19.7. Fermentation of lactose in whey GenBank M18108 contains the sequence for the promoter region and part of the coding sequence for LAC4. The vector is a derivative of pBR322 carrying the kanamycin (G418) resistance gene of Tn903 and ARS1B from Kluyveromyces lactis. This plasmid can replicate in E. coli, S. cerevisiae, and K. lactis. The order of the major features in this plasmid is: ampR - kanR - LAC12 - LAC4 > - ARS1B. |
| References |
Sreekrishna K, et al. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. Gene 28: 73-81, 1984. PubMed: 6329916
Sreekrishna K, Dickson RC. Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc. Natl. Acad. Sci. USA 82: 7909-7913, 1985. PubMed: 3934664
Leonardo JM, et al. Identification of upstream activator sequences that regulate induction of the beta-galactosidase gene in Kluyveromyces lactis. Mol. Cell. Biol. 7: 4369-4376, 1987. PubMed: 3125422
Dickson RC, Sreekrishna KK. Lac+ Saccharomyces cerevisiae. US Patent 5,047,340 dated Sep 10 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
