| 產品名稱 |
BM-N |
| 商品貨號 |
B177293 |
| Organism |
Bombyx mori, silkworm |
| Product Format |
frozen |
| Culture Properties |
mixed: adherent and suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
transfection host Host suitable for infection by Bombyx mori nuclear polyhedrosis virus (BmNPV), and may be used to propagate BmNPV-based expression vectors. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
The line should be cultivated at 27C. |
| Complete Growth Medium |
The base medium for this cell line is IPL-41 Insect Cell Culture Medium with glutamine (available from Sigma or Gibco). To make the complete growth medium, add the following components to the base medium:
- 2.6 g/L Tryptose Phosphate Broth (TPB)
- 0.069 mg/L ZnSO4·7H2O
- 3.86 mg/L AlCl3·6H2O
- 5 mg/L chlorophenol red
- 2g/L NaCl
- 10% heat-inactivated insect grade fetal bovine serum
Adjust pH to 6.3 to 6.4 by adding 1N NaOH
Adjust osmolarity to 317 to 360 by adding extra NaCl
The IPL-41 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation. (In Vitro 17: 495-509, 1981)
|
| Subculturing |
Medium Renewal: Once per week Gently resuspend attached cells in old culture medium by pipetting old medium across the cells on the floor of the flask. |
| Cryopreservation |
Culture medium, 90%; DMSO, 10% |
| Culture Conditions |
Temperature: 27.0°C |
| Name of Depositor |
Daiichi Seiyaku Co., Ltd. |
| Deposited As |
Bombyx mori |
| U.S. Patent Number |
|
| References |
Maeda S, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315: 592-594, 1985. PubMed: 2989694
. . J. Seric. Sci. Jpn. 53: 547-548, 1984.
Maeda S, Furusawa M. Method of producing substances in living silkworms. US Patent 5,118,616 dated Jun 2 1992
Maeda S, et al. Method of producing peptides using baculovirus vectors in cultured cells. US Patent 5,110,729 dated May 5 1992
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