Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 3.0; HindIII--6.8; KpnI--3.6, 3.2; PvuII--2.5, 2.4, 1.8; XbaI--5.0, 1.1, 0.65. The insert contains the following restriction sites (nt from the 5' end): MscI--227; KpnI--645; XbaI--1091, 1717; EcoRV--1608. A probe corresponding to nt 170-1135 detects mouse genomic restriction fragments of the following approximate sizes (kb): BamHI--3.8, BamHI/EcoRI--2.8; BglI--2.0; NcoI--6.2; KpnI--4.0, 0.7; The 5' 2.126 kb is sequenced, and contains the complete coding region (nt 267-1589). and detects a transcript of 8.5 kb. |
