| 產品名稱 |
pG7/T1 [N5-7/U7-T1] |
| 商品貨號 |
B177441 |
| Designations |
pG7/T1 [N5-7/U7-T1] |
| GenBank Number |
J02636
|
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
A Tzagoloff |
| Vector |
Construct size (kb): 14.39999961853027 |
| Insert |
DNA: genomic Insert lengths(kb): 6.699999809265137 Gene product: coenzyme QH2-cytochrome c reductase [COR1] |
| Insert Size (kb) |
6.700 |
| Genotype |
MATalpha ura3-1 cor1-1 |
| Mating Type |
alpha |
| Media |
ATCC® Medium 1688: Glycerol enriched medium
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
The insert contains the following sites separated by (kb) (approximate): EcoRI- 1.0- EcoRI- 0.1- SphI- 0.3- EcoRI- 1.0- XhoII- 1.6- EcoRI- 0.3- XbaI- 1.1- XhoI. Carries plasmid pG7/T1 with wild-type gene The smallest subclone capable of complementing a cor1-1 mutation was a 3.0 kb XhoII fragment. This region contains a continuous 1.368 kb open reading frame encoding a protein of 456 amino acids with a molecular weight of 50 kDa. The probe, pG7/T1, has been deposited in Escherichia coli RR1 as ATCC 37665 and in Saccharomyces cerevisiae N5-7/U7 as ATCC 66316. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
Tzagoloff A, et al. Assembly of the mitochondrial membrane system. Characterization of COR1, the structural gene for the 44-kilodalton core protein of yeast coenzyme QH2-cytochrome c reductase. J. Biol. Chem. 261: 17163-17169, 1986. PubMed: 3023384
|
