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Size (kb): 9.8999996185302730

Vector: pLKC480 (plasmid)

Promoters: Promoter none

Construction: A lacZY plasmid, Tn5

Marker(s):ampR,kanR

Construct size (kb): 9.899999618530273

Features: insert detection: lacZ
marker(s): ampR, kanR
promoter: none
replicon: pMB1
terminator: none
enhancer: none

contains easily purifiable cassette(s) for construction

integrating vector

vector permitting construction of fusion proteins

vector permitting visual detection of recombinants

The 6.3 kb lacZY-kanR cassette is released with SmaI.

Restriction digests of the clone give the following sizes (kb): EcoRI--10.05; BamHI--10.05; PstI--6.0, 4.4; HindIII--10.05.

One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the Escherichia coli genome.

A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid.

Temperature: 37.0°C