Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille
Depositors
Monsanto Co., FJ Perlak, Monsanto Co.
Applications
produces protein crystal protein (delta endotoxin)
Vector
Construct size (kb): 35.0
DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGS9 Intact vector size: 30.000 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI Polylinker sites: Construction: pACYC184 Host range: Escherichia coli Features (with orientation and position when available): replicon: P15A other: IS50L, <- marker(s): kanR, -> restriction site: BamHI other: IS50R, -> coding sequence: tra genes marker(s): cmlR Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: Y Strain: HD-1 Type of DNA: plasmid Insert end: BamHI Insert end: BamHI Insert size (kb): 4.6 Cross references:
Insert lengths(kb): 4.599999904632568
Gene product: crystal protein (delta endotoxin)
Target Gene: crystal protein (delta endotoxin)
Insert Size (kb)
4.600
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
The Tn5-B.t. gene segment (which conveys kanR) from pMAP29 was integrated into the host chromosome by mating E. coli HB101 containing pMAP29 with P. fluorescens 3732-3-7.
This strain is resistant to 100 mcg/ml rifampicin and 100 mcg/ml nalidixic acid.
Strain that proliferates in a plant environment to an extent sufficient to express at least 0.5 ng of insecticidally active protein per each insect to be controlled.
References
Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
