| 產(chǎn)品名稱 |
pHGH-1 [HGH X-1776] |
| 商品貨號 |
B177657 |
| Designations |
pHGH-1 [HGH X-1776] |
| GenBank Number |
I03167
|
| Species |
Homo sapiens, human |
| Depositors |
University of California, Davis |
| Applications |
For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). |
| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: GH1
Genomic copy number: unique
Gene name: growth hormone 1
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q22-q24
Tissue: pituitary neoplasm
Type of DNA: cDNA
Insert end: Modification: HindIII linkers
Insert end: Modification: HindIII linkers
Insert size (kb): 0.55
Cross references: DNA Seq. Acc.: I03167 Insert lengths(kb): 0.550000011920929 Tissue: pituitary neoplasm Gene product: growth hormone 1(growth hormone GH1) [GH1] |
| Insert Size (kb) |
0.550 |
| Media |
ATCC® Medium 1083: LB medium for chi 1776
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.5; PstI--4.5, 0.72. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). Contains sequences encoding aa 24-191 of the protein, plus 3' untranslated sequence. |
| References |
Goodman HM, et al. Recombinant DNA transfer vectors. US Patent 4,363,877 dated Dec 14 1982
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
