| 產品名稱 |
pALCA1SIFN |
| 商品貨號 |
B177797 |
| Designations |
pALCA1SIFN |
| Species |
Homo sapiens, human |
| Depositors |
Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V. |
| Applications |
in another host, produces protein interferon, alpha 2 |
| Vector |
Construct size (kb): 5.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pALCA1S
Intact vector size: 4.500
Type of vector: plasmid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: EcoRI, BamHI
Polylinker sites:
Other unique sites: HindIII
Construction: pDG6 (ATCCC 53169)
Host range: Escherichia coli; filamentous fungi
Features (with orientation and position when available):
promoter: alcA, ->
signal peptide coding region: synthetic, ->
MCS: XbaI...EcoRI
marker(s): ampR
replicon: pMB1
marker(s): ARGB
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNA2
Genomic copy number: unique
Gene name: interferon, alpha 2
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 p22
Type of DNA: cDNA
Insert 5' end: BglII
Insert 3' end: EcoRI
Insert size (kb): 0.9
Cross references: Insert lengths(kb): 0.8999999761581421 Gene product: interferon, alpha 2 [IFNA2] Target Gene: interferon, alpha 2 |
| Insert Size (kb) |
0.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--4.5, 0.9; HindIII--5.4, EcoRI--5.4. This plasmid may be difficult to isolate and digest. If this problem occurs, increase the final concentration of ampicillin in the growth mediun to 1 mg/ml. Transformants secrete protein, inducible by threonine and repressible by glucose. |
| References |
Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
