in another host, produces protein glucocorticoid receptor DNA binding factor 1
Vector
Construct size (kb): 10.60000038146973
DESCRIPTION OF VECTOR COMPONENT: Name of vector: pKCR-2 Intact vector size: 5.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: BamHI, EcoRI Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 replicon: pMB1 Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GRLF1 Gene name: glucocorticoid receptor DNA binding factor 1 Contains complete coding sequence?: Y Chromosome: 19; Localization: 19, 341.990000 cR Tissue: breast adenocarcinoma MCF7 cell line Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 4.9 Cross references: DNA Seq. Acc.: M73077
Insert lengths(kb): 4.900000095367432
Tissue: breast adenocarcinoma MCF7 cell line
Gene product: glucocorticoid receptor DNA binding factor 1 [GRLF1]
Target Gene: glucocorticoid receptor DNA binding factor 1
Insert Size (kb)
4.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglII--6.0, 4.6; EcoRI--5.4, 5.0; EcoRV--5.4, 2.9, 2.3; PvuII--10.5; SacI--6.0, 4.7; XbaI--4.7, 3.1, 2.4.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.47; BglII--1.67; EcoRV--1.84; NcoI--0.03, 2.14; NdeI--1.73; PstI--0.10, 1.82, 2.76; PvuII--0.55; SacI--0.72; SmaI--1.79; XbaI--2.30.
Contains the complete coding sequence (nt 34-2541) plus 1.5 kb 3' untranslated.
pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals.
References
LeClerc S, et al. Molecular Cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J. Biol. Chem. 266: 17333-17340, 1991. PubMed: 1894621
