| Comments |
The insert contains the following restriction sites (in bp from the 5' end of the complete cDNA): BamHI--2505, 2540, 3864, 3908; SphI--3083. Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7; HindIII--6.7; BamHI--4.4, 1.3, 1.0; PstI--6.7; XbaI--6.7. On its 3' end, Oc7 (ATCC 65038, 65039) overlaps Ac2 (ATCC 65040, 65041) by about 750 base pairs. On its 3' end, Ac2 overlaps HC-47 (ATCC 65042, 65043) by about 2400 base pairs. The insert encodes most of the collagenous domain and part of the non-collagenous domain. |
