| Comments |
Restriction digests of the clone give the following sizes (kb): SalI--12.0, 9.0, 8.0 doublet, 3.65; XhoI--41, 9.4; ClaI--35, 15; HindIII--23, 12, 5.5, 4.4; SalI/XhoI--9.0, 8.0, 7.5, 6.2, 5.5, 4.9, 3.35. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Insert includes the entire coding sequence as well as 5' and 3' flanking sequences on a 22 kb HpaI fragment. Promoter is either within or flanking the 5' end of the ApaI/XhoI fragment. Gene spans about 11 kb. |
| References |
Lin PF, et al. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol. Cell. Biol. 5: 3149-3156, 1985. PubMed: 3018504
Fiorentino L, et al. Assignment of the interleukin-2 locus to mouse chromosome 3. Genomics 5: 651-653, 1989. PubMed: 2613246
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