| Insert |
DNA: genomic Insert lengths(kb): 1.5 Tissue: DNA from hamster/human 20/X hybrid cell line Gene product: DNA Segment, single copy [D21S11] Alleles: B1, B1, B2, B2, A1, A1, A2, A2, E1, E1, E2, E2, E3, E3, E4, E4, E5, E5, E6, E6, E7, E7, E8, E8, E9, E9, E10, E10, E11, E11, E12, E12 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 1.4; HindIII--4.2; EcoRI--4.2; BamHI--uncut; XbaI--4.2. The TaqI RFLP is also detected by EcoRI. In that case, the B1 allele is 1.95 kb and the B2 allele is 2.95 kb. This clone is one of 3 unique HindIII/EcoRI fragments isolated from a single lambda clone, each of which detects RFLPs. The other clones are designated pG95alpha1-11b and pG95alpha1-11c. Enzyme(s) not detecting polymorphism: MspI, BglII, HaeIII, XbaI, HindIII, BamHI, BstEII, KpnI, CfoI, ClaII, PstI, SstI. |