| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--20.0, 12.0, 5.9, 4.8, 3.55; HindIII-->23.0, 6.8, 4.65, 4.5, 2.4; BglII--23.5, 10.0, 6.2, 2.7, 2.65; XbaI--31.5, 14.7. The Tn10 element contains the following restriction sites (nt from the IS10R end): EcoRI--3.14; HindIII--2.27, 6.99, 7.49; BamHI--6.14. Construct useful for general transposon mutagenesis in Escherichia coli. The order of the major features in this bacteriophage is: A-J-b221-cI857::Tn10 - O(UGA261). |
| References |
Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561
Kleckner N. DNA sequence analysis of Tn10 insertions: origin and role of 9 bp flanking repetitions during Tn10 translocation. Cell 16: 711-720, 1979. PubMed: 378398
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