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Construct size (kb): 5.52299976348877

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAST
Gene name: calpastatin
Contains complete coding sequence?: N
Chromosome: 5; Localization: 5 q15-q21
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.837
Cross references: DNA Seq. Acc.: D16217
Nucleotides ~1-1594 of the insert correspond to
nucleotides ~891-2486 of D16217.
DNA Seq. Acc.: U31345
Nucleotides 1-1837 of the insert correspond to
nucleotides 1-1837 of U31345.

Insert lengths(kb): 1.837000012397766

Tissue: placenta

Gene product: calpastatin [CAST]

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.8; BamHI--4.5; HindIII--2.8, 0.75, 0.55, 0.4; PstI--3.0, 1.3; EcoRI/PstI--2.7, 1.3, 0.6.

The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.68, 1.09, 1.66; PstI--1.20.