| 產品名稱 |
HHCMH17 |
| 商品貨號 |
B178666 |
| Designations |
HHCMH17 |
| GenBank Number |
M78844
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKMK1
Other gene symbols: MEK1
Gene name: protein kinase, mitogen-activated, kinase 1 (MAP kinase
kinase 1)
Contains complete coding sequence?: Y
Chromosome: 12
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: L11284; EST: EST00992; THC id: THC180162
Nucleotides 1-241 of the insert correspond to
nucleotides 803-1043 of L11284 with 100% identity.
DNA Seq. Acc.: L11284; EST: EST00993; THC id: THC180162
Nucleotides 1...300 of the insert correspond to
nucleotides 2138-1840 of L11284 with 96% identity.
DNA Seq. Acc.: M78844; EST: EST00992; Primer: M13 Forward;
THC id: THC180162
DNA Seq. Acc.: M78845; EST: EST00993; Primer: M13 Reverse;
THC id: THC180162 Insert lengths(kb): 1.5 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Gene product: polymyxin B resistance [MEK1] |
| Insert Size (kb) |
1.5 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI-3.0, 1.5. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; BamHI--4.4; HindIII--4.4; PstI--4.4; XbaI--4.4; XhoI--4.4. EST00992 matches PIR accession A32714 with 76.18% identity and 95.23% similarity over the sequence range: 3-65. |
| References |
Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
|
