Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; HindIII/XbaI--7.2, 1.8; SmaI--9.2; StuI--9.2. Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. Presence of alcohol dehydrogenase activity is used to follow cell lineage in culture or in situ. The order of the major features in this plasmid is: CMV promoter - Drosophila ADH-s - SV40 polyadenylation signal - RSV promoter - neoR - polyoma ori - SV40 ori - pMB1 ori. |
