| 產品名稱 | SU-DHL-8 |
|---|---|
| 商品貨號 | B179053 |
| Organism | Homo sapiens, human |
| Tissue | lymph node; derived from metastatic site: peritoneal effusion |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast-like |
| Culture Properties | suspension, grow as single cells and small clusters |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Large cell lymphoma |
| Age | 59 years |
| Gender | Male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Clinical Data | 59 years Caucasian Male |
| Receptor Expression | Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed) |
| Genes Expressed | Ig +; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa- |
| Comments | The depositor states that this cell line is negative for Epstein-Barr Virus (EBV). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Cultures can be maintained by the addition of fresh medium. Subculture the cells when cell density is ~ 1.0 x 106 to 2.5 x 106 cells/mL for higher viability. An inoculum of 1.0 x 105 to 4.0 x 105 cells/mL is recommended. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended. Medium renewal: Every 2 to 4 days (depending on cell density) |
| Cryopreservation | Storage temperature: liquid nitrogen vapor phase Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | D5S818: 11, 13 D13S317: 11, 13 D7S820: 8 D16S539: 12 CSF1PO: 11, 12 vWA: 15, 19 THO1: 6, 9 TPOX: 8 Amelogenin: X |
| Year of Origin | 1978 |
| References | Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
