Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--7.2, 2.1; BglII--6.0, 2.6; EcoRI--7.2, 1.5, 0.6. The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 7285 bp BamHI/HindIII fragment of YCp8 containing URA3 and CEN6 was ligated to a 1975 bp BamHI/HindIII fragment of YRp7-32phocat (ATCC 37625). |
