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Primary Bone Marrow CD34+ Cells, Normal, Human
Primary Bone Marrow CD34+ Cells, Normal, Human
規(guī)格:
貨期:
編號(hào):B179148
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Primary Bone Marrow CD34+ Cells, Normal, Human
商品貨號(hào) B179148
Organism Homo sapiens, human
Tissue Bone
Cell Type Hematopoietic stem
Morphology spherical; variable after culturing
Growth Properties suspension; variable after culturing
Biosafety Level 1  [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution 
All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.


Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age lot specific
Gender lot specific
Ethnicity lot specific
Applications Applications for use include the study of stem cell biology, hematology, transplantation, and oncology.
Product Format frozen 0.50 mL
Storage Conditions liquid nitrogen vapor phase
Comments

Cluster of differentiation 34 (CD34) is a sialomucin-family protein that is expressed on the surface of blood, endothelial, and bone marrow-derived progenitor cells. While the functions of CD34 are not well understood, there is evidence that the protein is involved in maintaining progenitor cells in an undifferentiated state.

The ATCC maintains CD34+ bone marrow-derived and CD34+ cord blood-derived cells which may be used to support physiologically relevant research in regenerative medicine, transplant studies, stem cell biology, hematology, and oncology. These pluripotent stem cells are isolated by immunomagnetic positive selection. In addition, the ATCC Cell Systems Laboratory has conducted in-depth characterization of the cells including differentiation into all three cell lineages.

Complete Growth Medium CD34+ cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining CD34+ cells in culture in the absence of application-specific growth factors.
Volume 0.50 mL
Cells per Vial One?vial?contains?a?minimum?of 5?x?105 ?viable?cells.
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV(I/II): Negative
HTLV(I/II): Negative
WNV: Negative
Trypanasome: Negative
Viability ≥ 70% when thawed from cryopreservation
C of A
Certificate of Analysis
Certificate of Analysis
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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