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Construct size (kb): 43.20000076293945

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: CBP2
Gene name: intron processing factor
Contains complete coding sequence?: Y
Chromosome: VIII; Localization: VIII L
Genome: Saccharomyces cerevisiae
Gene symbol: SSB1
Gene name: single-strand nucleic acid binding protein
Contains complete coding sequence?: Y
Chromosome: VIII
Genome: Saccharomyces cerevisiae
Gene symbol: RPL4A
Other gene symbols: MAK7
Gene name: ribosomal protein L7A-1
Contains complete coding sequence?: Y
Chromosome: VIII; Localization: VIII L, 44 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: GUT1
Gene name: glycerol kinase
Contains complete coding sequence?: Y
Chromosome: VIII; Localization: VIII L
Genome: Saccharomyces cerevisiae
Gene symbol: RIM1
Gene name: positive regulator of meiosis
Contains complete coding sequence?: Y
Chromosome: VIII
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 36.792
Cross references: DNA Seq. Acc.: U11583
Nucleotides 1-44587 of the insert correspond to
nucleotides 10483-55069 of U11583.

Insert lengths(kb): 36.79199981689453

Gene product: glycerol kinase [CBP2]

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--14.0, 5.0, 4.0 (doublet), 2.15, 1.8 (doublet), 1.5, 1.25 (triplet), 1.15 (doublet), 1.1 (doublet), 0.95 (doublet), 0.9 (doublet), 0.7 (triplet), 0.6 (doublet), 0.5.

Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 4.952, 4.182, 4.166, 2.253, 1.848, 1.848, 1.512, 1.247, 1.247, 1.241, 1.163, 1.163, 1.100, 1.100, 0.948, 0.912, 0.912, 0.869, 0.729, 0.713, 0.700, 0.616, 0.574, 0.477.

In an EcoRI/HindIII digest of the cosmid, the vector fragment is 13,365 kb.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Corresponds to nt 11143-47935 of the sequence of chromosome VIII.