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pPCC9-5
pPCC9-5
規格:
貨期:
編號:B179298
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pPCC9-5
商品貨號 B179298
Designations pPCC9-5
GenBank Number

M13572

Species Homo sapiens, human
Depositors RA Gravel
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.0
Tissue: SV40-transformed fibroblast
Gene product: propionyl Coenzyme A carboxylase, alpha polypeptide(propionyl CoA carboxylase, alpha polypeptide) [PCCA]
Alleles: A1, A2, B2, B1
Insert Size (kb) 2.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 2.0; HindIII--5.2; ClaI--5.2; PstI--3.9, 1.3.
Contains continuous open reading frame.
References

Bowcock AM, Hebert JM. The alpha chain of human propionyl CoA carboxylase (PCCA)(mapped to chromosome 13) detects an RFLP with XmnI. Nucleic Acids Res. 17: 8400, 1989. PubMed: 2573043

Lamhonwah AM, et al. Isolation of cDNA clones coding for the alpha and beta chains of human propionyl-CoA carboxylase: chromosomal assignments and DNA polymorphisms associated with PCCA and PCCB genes. Proc. Natl. Acad. Sci. USA 83: 4864-4868, 1986. PubMed: 3460076

Lamhonwah AM, Gravel RA. Propionicacidemia: absence of alpha-chain mRNA in fibroblasts from patients of the PCCA complementation group. Am. J. Hum. Genet. 41: 1124-1131, 1987. PubMed: 3687944

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