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Distribution host: Escherichia coli BL21(DE3)

Size (kb): 4.9429998397827150

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACYC177/ET3d
Intact vector size:
Type of vector: plasmid
Vector end: NcoI
Vector end: BamHI
Cloning sites: NcoI, NcoI+BamHI
Polylinker sites:
Construction: pACYC177,pET3d
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: p15A
marker(s): kanR, ->
ribosome-binding site: gene 10
promoter: T7 (phi10), ->
Cross references:

Vector: pACYC177/ET3d/yNMT (plasmid)

Promoters: Promoter for expression T7 (phi10)

Construction: pACYC177,pET3d

Marker(s):kanR

Construct size (kb): 4.900000095367432

Features: marker(s): kanR
promoter for expression: T7 (phi10)
replicon: p15A
coding sequence: NMT1

produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase

Restriction digests of the clone give the following sizes (kb): NdeI--5.5; NcoI--5.5; StuI--3.9, 1.5.

Expression system compatible with ColE1 plasmids, for cotransformation and production of myristoylated proteins.

Introduction of the NcoI site during amplification introduced a single base substitution T->G at nt 4.

Expression of this gene in the presence of 100 uM myristic acid allows myristoylation of recombinant proteins produced by a second ColE1 plasmid.

Temperature: 37.0°C