| 產品名稱 |
pHG50 |
| 商品貨號 |
B180021 |
| Designations |
pHG50 |
| Species |
Bos taurus, cow |
| Depositors |
Bio-Technology General Corporation, JP White, Bio-Technology General Corporation |
| Applications |
A HpaII fragment from pSK434 (ATCC 39784) containing lambda cI434 repressor was inserted in the ClaI site of pGH44 (ATCC 39806) to produce pHG50. |
| Vector |
Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHG44
Intact vector size: 4.600
Type of vector: plasmid
Vector end: HindIII
Vector end: NdeI
Cloning sites: HindIII NdeI
Polylinker sites:
Construction: pPS1, pRO 12
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: lambdaPL
terminator: T1T2
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: bovine
Gene symbol: GH
Gene name: growth hormone
Contains complete coding sequence?: U
Chromosome: 19; Localization: 19
Type of DNA: cDNA
Insert end: PstI
Insert end: EcoRI
Insert size (kb): 1.2
Cross references: Insert lengths(kb): 1.200000047683716 Gene product: growth hormone [GH] |
| Insert Size (kb) |
1.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): NdeI/HindIII--2.6, 2.3, 1.1, 0.8; PstI--4.4, 2.4; IcoRI--6.8; HindIII--4.9, 1.9; NdeI--3.4, 3.4. A HpaII fragment from pSK434 (ATCC 39784) containing lambda cI434 repressor was inserted in the ClaI site of pGH44 (ATCC 39806) to produce pHG50. |
| References |
Hartman JR, et al. Method for producing enzymatically active eucaryotic SOD in bacteria. US Patent 4,742,004 dated May 3 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
