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<em>Cunninghamella elegans</em> Lendner

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編號:B180290

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產品名稱	Cunninghamella elegans Lendner
商品貨號	B180290
Deposited As	Cunninghamella elegans Lendner
Strain Designations	PA-1
Application	Degrades 1-nitropyrene Degrades acenaphthene Degrades acridine Degrades dibenzothiophene Degrades fluoranthene Degrades petroleum crude oil Degrades phenanthrene Metabolizes 1-fluoronaphthalene Metabolizes 9,10-dimethylanthracene Metabolizes 9-hydroxymethylanthracene Metabolizes 9-methylanthracene Metabolizes fluorene Oxidizes dibenzothiophene Oxidizes isoquinoline Oxidizes quinoline Produces 3'-hydroxywarfarin Produces acridine trans-1,2-dihydrodiols Produces trans-dihydrodiols Transforms 1-nitrobenzo[e]pyrene Transforms benz[a]anthracene Transforms brompheniramine Transforms chlorpheniramine Transforms pheniramine Degrades phenanthrene to trans-dihydrodiols Degrades acridine to acridine trans-l,2-dihydrodiol and 2-hydroxyacridine Detoxification of 1-nitropyrene Oxidizes dibenzothiophene to dibenzothiophene sulphoxide and dibenzothiophene sulphone Transformation of benz[a]anthracene to trans-dihydrodiols
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Preceptrol®	no
Genome Sequenced Strain	Yes
Comments	Genome sequencing strain (Concordia University, Canada).
Morphology	After 10 days colonies spreading rapidly, at first white, later with dark spots of conidia, becoming gray, conidiophores erect, with verticillate or solitary branches; vesicles subglobose to pyriform, verrucose or smooth, conidia globose. 7-11 μm, or ovoid to ellipsoidal, 6-10 x 9-13 μm, smooth, verruculose, or shortly echinulate, hyaline, or with granular contents, brownish in mass.
Medium	ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 323: Malt agar medium ATCC^® Medium 336: Potato dextrose agar (PDA)
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTTATTCGGTCATTGGTTTTTATTCAAAAACCTTTGGCTTTAAATCATCCACAGTGTGGGAAATGTCTTCTAACGCTTGTGCCTGGTTCAGTCTAGTGCTGCCACTTGAGTTTACTCTTGGGTCAAGGGACCTTTGGGTAGTTTGTTCATTCGTGAGCAACCTCTTGTAACGGGGATAAGATTAATTTTATTATACTAAATTTTACTGAACTGATAGACCATAAATCTATGGTTGTTTTTTATTATAAACAAAAAAACAACTTTCAGCAATGGATCTCTCGGCTTTCGTATCGATGAAGAACGCAGCAAATCGCGATATGTAATGTGATCTGCCTATAGTGAATCATCAAATCTTTGAACGCATCTTGCACCTTATGGTATTCCATAAGGTACGTCTGTTTCAGTACCACTAGTAAATCTCCCCTCCACCTTGGTGGTTTAAAAGGAAGAGATAAATTATTACTGGTTCTGGTGATTCTTGATTTATTAAGAATTACTCTCGACCTAAATATAAGGCTCGACTTTTTTATTAGATCTCGCATCTGGTAAAACCTAGTCGGCTTTAATAGGATTTATTTCTTATTAGGTTTATAGCCATCATTTTACTTTTAAATCTTGGCCTGAAATCAGATGGGACTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTCCCCTAGTAACGGCGAGTGAAGAGGGAAAAGCTCAAAGTTGGAACCTGGTGGGCATAGCTCACCCGGATTGTAAACTAAAGTTTTTGAGTCGTTTAGTCAGCCAGGTAAATAAGTCCTCTGGAAAGGGGCGACATAGAGGGTGAAATCCCCGTCTTTGGCCTGAGTTTTGGTTAGGCGTTTGGCTTGGAAACGAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAAATGGGAGGTAAATCTCTCCTAAAGCTAAATATTGACGAAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGAAAAGAGGGTCAAAAAGTACGTGAAATTGCTGAAAGGGAACCGTATGAAATCAGACCTACTGGTAGGTAATCAATCTTTCCCTTGGGAAGGATGCACTTGCCTGCTATGTATGCCAGCGACATTTTGGTTGGGAGGAAAAAAATAGAAGGAATGTAGCCTAGGCTTCGGTTTAGGTGTTATAGACTTTTATAAAATACTCTCGGCTGGAATGAGGAACGCAGCAAACCGTAAGGCGAAGATTCTAGTCGCTTGGGGGGAATAATTAGAGAATTTCTGCTTCGGGTGGTGCTTTGATTATTACTTTCAACTCGGTTGGAGTTCTTTAATTTGCTTAGGTTGTTGGCTTAATGATTTATATGAC
Name of Depositor	JJ Perry
Isolation	Estuarine mud
Cross References	Nucleotide (GenBank) : Y17298 nucleotide and amino acid sequences of mRNA for enolase Nucleotide (GenBank) : AF195659 Cunninghamella elegans NADPH-dependent cytochrome P450 Nucleotide (GenBank) : Y17297 Cunninghamella elegans mRNA for 6-phosphogluconate dehydrogenase.
References	Perry JJ, Cerniglia CE. Effect of substrate on the fatty acid composition of hydrocarbon-utilizing filamentous fungi. J. Bacteriol. 118: 844-847, 1974. PubMed: 4829928 Cerniglia CE, et al. Fungal metabolism and detoxification of the nitropolycyclic aromatic hydrocarbon 1-nitropyrene. Appl. Environ. Microbiol. 50: 649-655, 1985. PubMed: 3907498 Crawford DL, Gupta RK. Oxidation of dibenzothiophene by Cunninghamella elegans. Curr. Microbiol. 21: 229-231, 1990. Cerniglia CE, et al. Effects of a fluoro substituent on the fungal metabolism of 1- fluoronaphthalene. Appl. Environ. Microbiol. 48: 294-300, 1984. PubMed: 6486779 Cerniglia CE, et al. Stereoselective fungal metabolism of methylated anthracenes. Appl. Environ. Microbiol. 56: 661-668, 1990. PubMed: 2317041 Pothuluri JV, et al. Fungal metabolism and detoxification of fluoranthene. Appl. Environ. Microbiol. 58: 937-941, 1992. PubMed: 1575497 Pothuluri JV, et al. Fungal metabolism of acenaphthene by Cunninghamella elegans. Appl. Environ. Microbiol. 58: 3654-3659, 1992. PubMed: 1482186 Pothuluri JV, et al. Biotransformation of fluorene by the fungus Cunninghamella elegans. Appl. Environ. Microbiol. 59: 1977-1980, 1993. PubMed: 8328814 Sutherland JB, et al. Enantiomeric composition of the trans-dihydrodiols produced from phenanthrene by fungi. Appl. Environ. Microbiol. 59: 2145-2149, 1993. Cerniglia CE, et al. Metabolism of benz[a]anthracene by the filamentous fungus Cunninghamella elegans. Appl. Environ. Microbiol. 60: 3931-3938, 1994. PubMed: 7993083 Wong YW, Davis PJ. Microbial models of mammalian metabolism: production of 3'- hydroxywarfarin, a new metabolite of warfarin using Cunninghamella elegans. J. Pharm. Sci. 80: 305-308, 1991. PubMed: 1865328 Schlenk D, et al. P450 catalysed S-oxidation of dibenzothiophene by Cunninghamella elegans. Xenobiotica 24: 1077-1083, 1994. PubMed: 7701849 Hansen EB Jr., et al. Fungal transformations of antihistamines: metabolism of brompheniramine, chlorpheniramine, and pheniramine to N-oxide and N- demethylated metabolites by the fungus Cunninghamella elegans. Xenobiotica 25: 1081-1092, 1995. PubMed: 8578764 Pothuluri JV, et al. Biotransformation of 1-nitrobenzo[e]pyrene by the fungus Cunninghamella elegans. J. Ind. Microbiol. Biotechnol. 22: 52-57, 1999. Wang RF, et al. Cloning, sequencing, and expression of the gene encoding enolase from Cunninghamella elegans. Mycol. Res. 104: 175-179, 2000. Cerniglia CE, Perry JJ. Crude oil degradation by microorganisms isolated from the marine environment. Z. Allg. Mikrobiol. 13: 299-306, 1973. PubMed: 4797813 Sutherland JB, et al. Identification of metabolites produced from acridine by Cunninghamella elegans. Mycologia 86: 117-120, 1994. Sutherland JB, et al. N-Oxidation of quinoline and isoquinoline by Cunninghamella elegans. Exp. Mycol. 18: 271-274, 1994. Kim YH, et al. Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans. Arch Biochem Biophys 494: 159-165, 2010. PubMed: 19944668

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