| 產品名稱 |
28-2-7 |
| 商品貨號 |
B180731 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The 28-2-7 (ATCC CRL-2487) and 28-6-20 (ATCC CRL-2489) antibodies bind the V kappa 24 light chain idiotypic specificity of MOPC167. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. The antibodies are specific for PC-binding proteins of VH1/V kappa 24 heavy and light chains of various isotypes. These antibodies can be used as typing reagents for mouse IgA bearing the V kappa 24 idiotypic specificity. They can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of the IgA PC specific MOPC167 myeloma. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against MOPC167 idiotype (V kappa 24) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against MOPC167 idiotype (V kappa 24) |
| Comments |
The 28-2-7 (ATCC CRL-2487) and 28-6-20 (ATCC CRL-2489) antibodies bind the V kappa 24 light chain idiotypic specificity of MOPC167. Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibodies are specific for PC-binding proteins of VH1/V kappa 24 heavy and light chains of various isotypes. They do not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins. These antibodies can be used as typing reagents for mouse IgA bearing the V kappa 24 idiotypic specificity. They can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of the IgA PC specific MOPC167 myeloma. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
|
| Subculturing |
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) cells/ml. Interval: Maintain cultures at a cell concentration between 5 X 10(4) and 5 X 10(5) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG1 kappa |
| Name of Depositor |
DG Sieckmann |
| Deposited As |
rat (B cell); mouse (myeloma) |
| Year of Origin |
1990 |
| References |
Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702
|
