| 產品名稱 |
pHP11 [UTR-9853] |
| 商品貨號 |
B181271 |
| Designations |
pHP11 [UTR-9853] |
| GenBank Number |
M81457
|
| Species |
Homo sapiens, human |
| Depositors |
TP Dooley |
| Vector |
Construct size (kb): 3.599999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAL1L
Gene name: calpactin I, light polypeptide (p11)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1, cR
Tissue: foreskin keratinocyte
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: HindIII
Insert size (kb): 0.55
Cross references: DNA Seq. Acc.: M81457
Nucleotides 1-545 of the insert correspond to
nucleotides 1-545 of M81457. Insert lengths(kb): 0.550000011920929 Tissue: foreskin keratinocyte Gene product: calpactin I, light polypeptide (p11) [CAL1L] |
| Insert Size (kb) |
0.550 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.6; ScaI--2.2, 1.5; NcoI--3.6; XmnI--1.9, 1.5; BamHI--3.5. The insert contains the following restriction sites (approximate kb from the 5' end): NcoI--0.1; ScaI--0.17; SphI--0.32; XmnI--0.46. Corresponds to nt 1-545 of the sequence record, including the complete coding region (nt 72-365). The 5' end is linker derived; the 3' end is a naturally occurring site. |
| References |
Dooley TP, et al. cDNA sequence of human p11 calpactin I light chain. Genomics 13: 866-868, 1992. PubMed: 1386341
Thomas P Dooley, personal communication
|
