| 產(chǎn)品名稱 | Influenza A virus (H1N1) |
|---|---|
| 商品貨號 | B181285 |
| Classification | Orthomyxoviridae, Influenzavirus A |
| Deposited As | Influenza A virus |
| Agent | Influenza A virus (H1N1) |
| Strain | A/WS/33 |
| Applications | Influenza Research |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | Derived by adaptation of egg allantoic fluid product ATCC® VR-825™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-825™ and ATCC® VR-1520™ have not been compared for sequence or infectivity in eggs and tissue culture. Demonstrates cross-reactivity with Influenza A virus, A/PR/8/34 and swine influenza. |
| Effect on Host | CPE, cell rounding and sloughing |
| Recommended Host | MDCK (ATCC® CCL-34™) |
| Growth Conditions | Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
|
| Name of Depositor | WA Chappell, Centers for Disease Control and Prevention; ATCC |
| Special Collection | NCRR Contract |
| Source | Throat washings from an influenza patient |
| References | Burnet FM. A genetic approach to variation in influenza viruses; the characters of three substrains of influenza virus A (WS). J. Gen. Microbiol. 5: 46-53, 1951. PubMed: 14824469 Smith W, et al. A virus isolated from influenza patients. Lancet 2: 66-68, 1933. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
