Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
organically enriched sediment sample from intertidal zone, south of island cliffs, Appledore Island, ME, 1993
Product Format
test tube
Type Strain
yes
Comments
species description
Medium
ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 25.0°C
Duration: anaerobic
Protocol: ATCCNO: 50416 SPEC: Upon receipt aseptically reduce the volume of the culture to 12 ml by removing approximately 2 ml of fluid. Screw the cap on tightly and incubate at 25C on a 5-15 degree horizontal slant. When the bacteria have been largely cleared from the culture (1-2 days), gently invert the culture several times and aseptically transfer 0.1 - 0.3 ml to a fresh tube of ATCC medium 1525 (12 ml of medium in a 16 x 125 mm screw-capped test tube) bacterized with Enterobacter aerogenes ATCC-13048. The medium is bacterized by inoculating a loopful of bacteria into a fresh tube of medium at least 24 hours prior to inoculation with the ciliate.
Subcultivation
Protocol: ATCCNO: 50416 SPEC: Upon receipt aseptically reduce the volume of the culture to 12 ml by removing approximately 2 ml of fluid. Screw the cap on tightly and incubate at 25C on a 5-15 degree horizontal slant. When the bacteria have been largely cleared from the culture (1-2 days), gently invert the culture several times and aseptically transfer 0.1 - 0.3 ml to a fresh tube of ATCC medium 1525 (12 ml of medium in a 16 x 125 mm screw-capped test tube) bacterized with Enterobacter aerogenes ATCC-13048. The medium is bacterized by inoculating a loopful of bacteria into a fresh tube of medium at least 24 hours prior to inoculation with the ciliate.
Name of Depositor
EB Small
Chain of Custody
ATCC <<--EB Small<<--S.A. Schaffer
Year of Origin
1993
References
Nerad TA, et al. Trimeya shoalsia sp. n., an anaerobic microaerotolerant marine cillitate from Aplledore Island, Gulf of Maine, USA. Acta Protozool. 34: 289-298, 1995.
