Restriction digests of the clone give the following sizes (kb): BamHI--5.1; EcoRI--5.1; BamHI/EcoRI--4.4, 0.7; consistent with deposited DNA.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Chedid M, Mizel SB. Involvement of cyclic AMP-dependent protein kinases in the signal transduction pathway for interleukin-1. Mol. Cell. Biol. 10: 3824-3827, 1990. PubMed: 2192264
Tanaka T. Restriction of plasmid-mediated transformation in Bacillus subtils 168. Mol. Gen. Genet. 175: 235-237, 1979. PubMed: 117281
