| 產(chǎn)品名稱 |
pGM30 |
| 商品貨號(hào) |
B182174 |
| Designations |
pGM30 |
| Depositors |
OmniGene, Inc., RR Yocum, OmniGene, Inc. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli YMC9 |
| Vector Information |
Size (kb): 8.8000001907348630 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGM30
Intact vector size: 8.800
Type of vector: plasmid
Vector end: XhoI
Vector end: XbaI
Cloning sites: XbaI XhoI
Polylinker sites:
Construction: pRY271, pGM11
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3
promoter: TPI
leader peptide: DEX4
coding sequence: DEX4
transcription terminator: TPI
replicon: pMB1
marker(s): ampR
replicon: 2 micron
Cross references: Vector: pGM30 (plasmid) Promoters: Promoter TPI Construction: pRY271, pGM11 Marker(s):URA3,ampR Construct size (kb): 8.800000190734863 Features: leader peptide: DEX4
marker(s): URA3
marker(s): ampR
promoter: TPI
replicon: 2 micron
replicon: pMB1
transcription terminator: TPI
coding sequence: DEX4 |
| Applications |
expression vector produces protein glucoamylase DEX4 variant shuttle vector vector with sequences for protein export glucoamylase DEX4 variant |
| Comments |
Restriction digests of the clone give the following sizes (kb): XbaI/XhoI--7.2, 3.2; SmaI--10.0; XbaI--10.0. A shuttle expression vector containing the DEX4 glucoamylase (GA) variant of Saccharomyces cerevisiae or S. diastaticus that encodes a sporulation-specific GA. The DEX4 signal sequence is very useful in the production of secreted fusion proteins. To use pGM30 as a secretion expression vector, remove the 2.7 kb XhoI/XbaI fragment (which contains all of DEX4 except for the upstream signal sequence). The replacement insert should have a 5' XhoI end and a 3' XbaI end for proper orientation. When transformed into yeast strain RD61-1D, pGM30 yielded approximately 70 units of glucoamylase activity. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Maine GT, et al. Expression and secretion vector for yeast containing a glucoamylase signal sequence. US Patent 5,024,941 dated Jun 18 1991
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
