| 產品名稱 |
pE25.a [pBS300] |
| 商品貨號 |
B182196 |
| Designations |
pE25.a [pBS300] |
| Depositors |
IE Scheffler |
| Vector |
Vector: pE25.a
Size: 3,200 bp
Selectable Marker: ampicillin resistant
Cloning sites: EcoR I, Sac I, Kpn I, Sma I, BamHI, Xba I, Sal I, Pst I, Sph I, Hind III
Replicon: pUC (pMB1)
Features: Lac, T3 and T7 promoters |
| Insert |
DNA: genomic
Insert lengths(kb): 0.349
Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product: DNA Segment, repetitive [D1Z7] |
| Insert Size (kb) |
0.349 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Plasmid in Escherichia coli |
| Comments |
The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227). Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6. The first half of the dimer is 85% identical to the sequence of the 1' monomer; the second half is 80% identical to the 2' monomer. The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof. |
| References |
Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415
Immo E Scheffler, personal communication
|
