人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

produces protein insulin

Construct size (kb): 10.60000038146973

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: EcoRI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

DNA: Synthetic

DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: INS
Genomic copy number: unique
Gene name: insulin
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 p15.5
Type of DNA: synthetic
Insert end: EcoRI
Insert end: BamHI
Insert size (kb): 0.1
Cross references:

Insert lengths(kb): 0.1000000014901161

Gene product: insulin [INS]

Alleles: K2, A3, B2, A1, A1, A4, A3, A3, A5, A6, B1, K1, A1, A2, A2, A2

Target Gene: insulin

Restriction digests on an agarose gel give the following sizes (kb): EcoRI--6.4, 4.1; BamHI--10.5.

The sequence of the insert is AATTCATGTT CGTCAATCAG CACCTTTGTG GTTCTCACCT CGTTGAAGCT TTGTACCTTG TTTGCGGTGA ACGTGGTTTC TTCTACACTC CTAAGACTTA ATAGGATC, including a HindIII site at position 46.

This construct directs the synthesis of a beta-galactosidase-insulin B chain fusion protein regulated by the lac promoter. The B peptide can be released by cyanogen bromide cleavage and used to reconstitute insulin with the product of pIA1, ATCC 31448.

This clone was constructed from pBR322, an EcoRI fragment from lambda plac containing most of the beta-galactosidase gene, and synthetic DNA encoding human insulin B chain.