| 產品名稱 |
p67-10 |
| 商品貨號 |
B183442 |
| Designations |
p67-10 |
| GenBank Number |
M32011
|
| Species |
Homo sapiens, human |
| Depositors |
TL Leto |
| Applications |
produces protein neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) neutrophil cytosolic factor 2 |
| Vector |
Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NCF2
Genomic copy number: unique
Gene name: neutrophil cytosolic factor 2 (65kD, chronic granulomatous
disease, autosomal 2)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 cen-q32
Tissue: HL-60 cell line (promyelocytic), differentiated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.16
Cross references: DNA Seq. Acc.: M32011 Insert lengths(kb): 2.160000085830689 Tissue: HL-60 cell line (promyelocytic), differentiated Gene product: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)(neutrophil cytosolic factor 2) [NCF2] Alleles: A1, A2 Target Gene: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) |
| Insert Size (kb) |
2.160 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1; SstI--3.7, 0.9, 0.41; BamHI--4.0, 1.0; BglII--5.1; PstI--5.1. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.84, 1.22; BamHI--0.97; BglII--0.78; SmaI--1.17. Contains the complete coding sequence (nt 68-1648). Detects a transcript of 2.4 kb. The polymorphism was detected with a 0.84 kb EcoRI/SacI fragment. Enzymes(s) not detecting polymorphism: AvaI, BamHI, EcoRI, KpnI, PstI, SacI, XhoI. |
| References |
Francke U, et al. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. PubMed: 2393022
Leto TL, et al. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60(c-src). Science 248: 727-730, 1990. PubMed: 1692159
Kenney RT, Leto TL. A HindIII polymorphism in the human NCF2 gene. Nucleic Acids Res. 18: 7193, 1990. PubMed: 1979859
|
