Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.73; PstI--3.7; BamHI--3.2, 0.55; PvuI--2.6, 1.2; AvaI--3.75. The insert encodes the complete 222 amino acid MnSOD precursor and contains 91 bp of 5' untranslated and 21 bp of 3' untranslated sequence, and poly(A). There are 2 TaqI polymorphisms which exhibit some linkage disequilibrium; the pairwise linkage disequilibrium value is 0.6 for Caucasians. Enzyme(s) not detecting polymorphism: BamHI, BglII, KpnI, RsaI. The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.13, 0.24; BamHI--0.6. |
