| 產品名稱 |
3T3-L1 MBX |
| 商品貨號 |
B183606 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
adipogenesis and adipocyte differentiation, diabetes and obesity research areas |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
3T3 L1-MBX clone was derived from 3T3-L1 (CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin |
| Comments |
The 3T3 L1-MBX clone was derived from 3T3-L1 (ATCC CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin. In addition, 3T3 L1-MBX has a great insulin-stimulated glucose uptake response which is about 8-10 fold window with sub-maximal insulin concentration in 2-deoxyglucose uptake assay (2-DOG). This cell line would be a valuable tool for researchers who are interested in diabetes and obesity research areas. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Note: Never allow culture to become completely confluent.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
The recommended inoculum is 3 to 5 X 103 cells/cm2. Subculture before cultures become 80 to 90% confluent.
Interval: Every three days
Medium Renewal: 2 to 3 times per week
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| Cryopreservation |
90% FBS supplemented with 10% DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
CymaBay Therapeutics (formerly Metabolex, Inc.) & Choi Y |
| References |
Gregoire FM, et al. MBX-102/JNJ39659100, a novel peroxisome proliferator-activated receptor-ligand with weak transactivation activity retains antidiabetic properties in the absence of weight gain and edema. Mol. Endocrinol. 23(7): 975-88, 2009. PubMed: 19389808
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