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Size (kb): 8.5000000000000000

Vector: pDdGal-15 (plasmid)

Promoters: Promoter none

Construction: pDlac-1, pB10TP2

Marker(s):G418R,ampR

Construct size (kb): 8.5

Features: insert detection: lacZ
marker(s): ampR, G418R
promoter: none
replicon: pMB1
terminator: actin 8

promoter-cloning vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; BamHI--8.5; BglII--8.5.

A promoter-cloning plasmid vector using expression of beta-galactosidase as the reporter in Dictyostelium.

pDdGal-15 (ATCC 37770), pDdGal-16 (ATCC 37771) and pDdGal-17 (ATCC 37772) differ in their polylinker sequences. Each contains a BglII site in a different reading frame relative to the lacZ gene.

The lacZ gene and Dictyostelium actin 8 terminator from pDdlac-1 were inserted into a derivative of pB10TP2 from which the f1 origin and and a lacZ fragment had been removed.

The neo gene is regulated by an actin 6 promoter and a CP1 terminator.

Temperature: 37.0°C