| Insert |
DNA: cDNA Insert lengths(kb): 3.099999904632568 Tissue: brain stem Gene product: glutamate receptor, ionotropic, AMPA 1(glutamate receptor 1, glutamate receptor, kainate) [GLUR1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 2.9; PvuII--2.4, 2.35, 0.6, 0.3; PstI--5.1, 0.55, 0.45; BamHI--6.1. The insert contains the following restriction sites (nt from the 5' end): PvuII--447, 568, 837; PstI--464, 551, 1100, 1248; BglII--1969. Contains the complete coding region (for a product of 907 aa). Detects a mRNA of 5.5 kb in human brain stem and cerebellum. The 5' end of the insert is next to the T7 promoter. |
| References |
Puckett C, et al. Molecular cloning and chromosomal localization of one of the human glutamate receptor genes. Proc. Natl. Acad. Sci. USA 88: 7557-7561, 1991. PubMed: 1652753
Carmie Puckett, personal communication
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