| 產品名稱 |
CrmA T291R |
| 商品貨號 |
B184319 |
| Designations |
CrmA T291R |
| Species |
Cowpox virus |
| Depositors |
G Salvesen |
| Applications |
produces protein cytokine response modifier A, inactive mutant |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pFlag-1 deriv
Intact vector size:
Type of vector: phagemid
Vector end: NcoI
Vector end: SalI
Cloning sites: HindIII EcoRI XbaI SalI BglII XhoI
Polylinker sites: HindIII EcoRI XbaI SalI BglII XhoI
Construction: pFLAG, FLAG/ompA sequences deleted
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: tac
replicon: pMB1, f1
repressor gene: lacI
transcription terminator: rrnB
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Cowpox
Gene symbol: CrmA
Gene name: cytokine response modifier A
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.2
Cross references: Insert lengths(kb): 1.200000047683716 Gene product: cytokine response modifier A [CrmA] Target Gene: cytokine response modifier A, inactive mutant |
| Insert Size (kb) |
1.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): NcoI/SalI--6.1, 0.85; PstI--4.2, 1.8, 0.9; BamHI--6.5, 0.45. Insert encodes an inactive control protein tagged with hexahistidine at the N-terminus. The T291R mutation occurs within the reactive site loop. |
| Classification |
Poxviridae, Orthopoxvirus |
| References |
Tewari M, et al. Yama/CPP32beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801-809, 1995. PubMed: 7774019
|
