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pAQP1 [UTR-01749, WI-9113, pCHIP]
pAQP1 [UTR-01749, WI-9113, pCHIP]
規格:
貨期:
編號:B184422
品牌:Mingzhoubio

標準菌株
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DNA
RNA

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甘油
平板


產品名稱 pAQP1 [UTR-01749, WI-9113, pCHIP]
商品貨號 B184422
Designations pAQP1 [UTR-01749, WI-9113, pCHIP]
GenBank Number

M77829

Species Homo sapiens, human
Vector
Construct size (kb): 5.760000228881836
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->, 3-459
primer site: M13-20, ->
promoter for in vitro transcription: T7, ->
insert detection: lacZ', <-, 816-938
MCS: SacI...KpnI, ->, 657-759
promoter for in vitro transcription: T3, <-
primer site: reverse, <-
replicon: pMB1, 1032-1972
marker(s): ampR, <-, 1975-2832
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AQP1
Other gene symbols: CHIP28
Gene name: aquaporin 1 (channel-forming integral protein, 28 kD)
Contains complete coding sequence?: N
Chromosome: 7; Localization: 7 p14; 7, 84.8799999 cR
Tissue: bone marrow
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 2.8
Cross references: DNA Seq. Acc.: M77829
Nucleotides 1-1283 of the insert correspond to
nucleotides 53-1335 of M77829.
Insert lengths(kb): 2.799999952316284
Tissue: bone marrow
Gene product: aquaporin 1 (channel-forming integral protein, 28 kD) [AQP1]
Insert Size (kb) 2.800
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.8; HindIII--4.4, 1.5; SmaI--5.0, 0.8; KpnI--5.8; BglII--5.8.
The insert contains the following restriction sites (approximate kb from the 5' end): SmaI--0.80; HindIII--1.28.
The insert begins at nt +15 relative to the initiation ATG, and contains about 1.3 kb at the 3' end that has not been sequenced.
References

Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319

Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524

Preston GM, et al. The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel. J. Biol. Chem. 268: 17-20, 1993. PubMed: 7677994

Bondy C, et al. Developmental gene expression and tissue distribution of the CHIP28 water-channel protein. Proc. Natl. Acad. Sci. USA 90: 4500-4504, 1993. PubMed: 8506291

Moon C, et al. The human aquaporin-CHIP gene. J. Biol. Chem. 268: 15772-15778, 1993. PubMed: 8340403

Preston GM, et al. Membrane topology of aquaporin CHIP. J. Biol. Chem. 269: 1668-1673, 1994. PubMed: 7507481

Jung JS, et al. Molecular structure of the water channel through aquaporin CHIP. J. Biol. Chem. 269: 14648-14654, 1994. PubMed: 7514176

梅經理 17280875617 1438578920
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周經理 17757487661 1296385441
于經理 18067160830 2088210172
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