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Size (kb): 8.6999998092651370

Vector: pUFR034 (cosmid)

Promoters: Promoter lac

Construction: pUC18, pSa747, pHC79, pUCD550, pUC4Kan, pLAFR3

Marker(s):neoR

Construct size (kb): 8.699999809265137

Features: insert detection: lacZ'
marker(s): neoR
promoter: lac
replicon: oriW

shuttle vector

vector for constructing genomic libraries

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): EcoRI--8.7; PstI--6.4, 1.35, 1.2; SmaI--7.0, 1.8.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Uses interruption of lacZalpha.

Shuttle vector permitting visual detection of recombinants.

The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture.

The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - cos (pHC79) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+).

Temperature: 30.0°C