DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK+ Intact vector size: 2.964 Type of vector: phagemid Vector end: SmaI closer to PstI site Vector end: SmaI Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ' marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references:
Vector: pPluc* (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript SK+, pUHC131-1
Marker(s):ampR
Construct size (kb): 4.699999809265137
Features: insert detection: lacZ' marker(s): ampR promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: Kpn...SacI coding sequence: luc*
Applications
contains easily purifiable cassette(s) for construction luciferase, modified
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.0, 1.7; BamHI/BstEII--3.6, 1.15; BamHI/NcoI--3.0, 1.7.
One of two vectors (ATCC 87108 and 87109) containing a luciferase gene altered to conserve amino acid sequence and function but to remove internal restriction sites to facilitate excision.
These constructs differ in their orientation. The sites listed in the polylinker (MCS) can be used to excise the gene for insertion into another plasmid.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Bonin AL, et al. Photinus pyralis luciferase: vectors that contain a modified luc coding sequence allowing convenient transfer into other systems. Gene 141: 75-77, 1994. PubMed: 8163178
