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貨號：B245025
名稱：Acanthamoeba healyi Moura et al.

貨號：B245024
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245023
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245022
名稱：Eutypella vitis (Schweinitz) Cooke emend. Glawe

貨號：B245021
名稱：Streptomyces scabiei (Thaxter) Lambert and Loria

貨號：B245020
名稱：Graphium cuneiferum (Berkeley et Broome) Mason et Ellis

貨號：B245019
名稱：HFBCI28

貨號：B245018
名稱：Septoria passiflorae Sydow, anamorph

貨號：B245017
名稱：Dictyostelium purpureum Olive

貨號：B245016
名稱：Y71

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pECV25

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編號:B186653

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產品名稱	pECV25
商品貨號	B186653
Designations	pECV25
Depositors	C Backendorf
Biosafety Level	1
Vector Information	Size (kb): 11.3170003890991200 Vector: pECV25 (plasmid) Promoters: Promoter RSV LTR Construction: pRSVneo, p205 Marker(s):G418R,ampR,hygR Construct size (kb): 11.31700038909912 Features: marker(s): ampR, hygR, G418R promoter: RSV LTR replicon: pMB1, EBV oriP terminator: beta-globin polyadenylation
Applications	contains sequence nuclear antigen 1 expression vector shuttle vector
Comments	Restriction digests of the clone give the following sizes (kb): HindIII/SacI--5.7 (doublet); SalI--11.5; XhoI--11.5. Shuttle expression vector maintained episomally in human cells at a copy number of 25 - 50. Shows high transfection efficiency and can be introduced as supercoiled plasmids. Vector can be rescued from transfected cells with no detectable rearrangements. The EBNA-1 gene contains an in-frame deletion of a repetitive region of 700 +/- 20 bp. This deletion increases the number of stably maintained plasmid molecules per cell. The order of the major features in this plasmid is: bla (ampR) - hph (hygR) - RSV LTR - HindIII - rabbit beta-globin intron splicing signals - NotI - agpt (G418R) - XhoI - rabbit beta-globin polyadenylation signal - SalI - oriP - SacI - EBNA-1 - ClaI.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Belt PB, et al. Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus-derived cDNA expression vector. Nucleic Acids Res. 19: 4861-4866, 1991. PubMed: 1656380 Belt PB, et al. Induction of a mutant phenotype in human repair proficient cells after overexpression of a mutated human DNA repair gene. Nucleic Acids Res. 19: 5633-5637, 1991. PubMed: 1945841 Belt PB, et al. Construction and properties of an Epstein-Barr-virus-derived cDNA expression vector for human cells. Gene 84: 407-417, 1989. PubMed: 2482230

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