| 產品名稱 |
pBIIOI [NEB 532] |
| 商品貨號 |
B187214 |
| Designations |
pBIIOI [NEB 532] |
| Depositors |
New England Biolabs, Inc., PA Rees, New England Biolabs, Inc. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 4.4000000953674320 Vector: pBIIOI (plasmid) Promoters: Promoter none Construction: pBR322 Marker(s):ampR,tetR Construct size (kb): 4.400000095367432 Features: marker(s): ampR, tetR
promoter: none
replicon: pMB1
terminator: none
enhancer: none |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; BglII--4.4; EcoRI/PstI--3.8, 0.7; HindIII--4.4; EcoRI/PvuII--2.3, 2.1. General purpose cloning vector. Derivative of pBR322 with a BglII linker inserted at the EcoRI site. The order of the major features in this plasmid is: EcoRI - BglII - EcoRI - ClaI - HindIII - tet - PvuII - ori - bla. |
| Media |
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Rees PA, Brenner JS II. Method for producing the HincII restriction endonuclease and methylase. US Patent 5,015,581 dated May 14 1991
Phyllis A Rees, personal communication
|
| Shipped |
freeze-dried |
