| Vector |
Construct size (kb): 7.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19
Intact vector size: 2.900
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MYBL1
Gene name: avian myeloblastosis viral (v-myb) oncogene homolog-like 1
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 q22
Tissue: B-lymphocytes
Type of DNA: cDNA
Insert 5' end: BamHI
Insert 3' end: EcoRI
Insert size (kb): 4.9
Cross references: DNA Seq. Acc.: X13294
Nucleotides 1-2317 of the insert correspond to
nucleotides 25-2341 of X13294.
DNA Seq. Acc.: X66087
Nucleotides 1270-4857 of the insert correspond to
nucleotides 1-3587 of X66087. Insert lengths(kb): 4.900000095367432 Tissue: B lymphocyte Gene product: avian myeloblastosis viral (v-myb) oncogene homolog-like 1 [MYBL1] Target Gene: avian myeloblastosis viral (v-myb) oncogene homolog-like 1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.6, 3.0, 0.62, 0.35, 0.21, 0.19; EcoRI--3.6(doublet), 0.35, 0.23, 0.19; HindIII--4.8, 3.1; PstI--5.3, 2.4, 0.21; PvuII--4.3, 2.6, 1.1. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.13; BglII--1.74; EcoRI--0.64, 0.96, 1.11, 1.29; EcoRV--2.31; HindIII--3.11; KpnI--1.75, 3.45; NdeI--0.75; NcoI--1.31; PstI--2.40, 2.69; PvuII--0.93; SacI--2.35. There are internal EcoRI sites. The coding portion of the insert can be excised with HindIII. |
| References |
Golay J, et al. The human A-myb protein is a strong activator of transcription. Oncogene 9: 2469-2479, 1994. PubMed: 8058310
Josee Golay, personal communication
|
