| 產品名稱 |
pv-kit |
| 商品貨號 |
B188425 |
| Designations |
pv-kit |
| Species |
feline sarcoma virus (type C) |
| Depositors |
P Besmer |
| Vector |
Construct size (kb): 3.400000095367432 |
| Insert |
DNA: genomic Insert lengths(kb): 0.699999988079071 Gene product: Hardy-Zuckerman 4 feline sarcoma viral (v-kit) oncogene homolog( Mast cell growth factor, c-kit proto-oncogene protein) [W] Alleles: A1, A2, B1, B2 |
| Insert Size (kb) |
0.700 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): SacI--3.4; SacI/SalI--2.7, 0.68. The v-kit specific probe (0.7 kb SacI/SalI fragment) hybridizes strongly to human sequences and more weakly to mouse sequences. Contains nucleotides 1254-1959 of gag-kit of HZ4-FeSV (Hardy-Zuckerman 4 feline sarcoma virus). |
| References |
Curtis D. An SstI RFLP at the c-kit oncogene locus (KIT). Nucleic Acids Res. 19: 1352, 1991. PubMed: 1709489
Besmer P, et al. A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family. Nature 320: 415-421, 1986. PubMed: 3007997
Berdahl LD, et al. A HindIII RFLP demonstrated for the kit oncogene on chromosome 4. Nucleic Acids Res. 16: 4740, 1988. PubMed: 2898128
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