| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 16.0, 9.2, 2.8; EcoRI--20.0, 14.0, 9.2, 5.6; HindIII--23.0, 10.0, 7.8, 1.6; SacI--20.0, 9.2, 5.6, 4.6, 2.8, 2.5, 1.0 (doublet); XhoI--20.0, 14.0, 9.2, 5.0. The insert contains the following restriction sites (approximate kb from the 5' BamHI site): BamHI--2.8, 18.7; HindIII--9.5, 17.5; EcoRI--13.7. A 0.6 kb XhoI/PstI fragment has been used as an alpha-specific probe. The 3.8 kb EcoRI/HindIII fragment contains sequences characteristic of the A2m(2) allotype constant region and its 5' flanking sequences. The 1.1 kb HindIII/BamHI fragment contains the transmembrane exon. The insert begins just 5' to the sequences encoding the epsilon constant region and extend approximately 4.3 kb beyond the exons for the alpha chain. Additional GenBank/EMBL accession numbers: M35287, M35291. |
| References |
Lefranc MP, Rabbitts TH. Human immunoglobulin heavy chain A2 gene allotype determination by restriction fragment length polymorphism. Nucleic Acids Res. 12: 1303-1311, 1984. PubMed: 6322103
Bensmana M, Lefranc MP. Gene segments encoding membrane domains of the human immunoglobulin gamma 3 and alpha chains. Immunogenetics 32: 321-330, 1990. PubMed: 1979064
Flanagan JG, et al. Mechanisms of divergence and convergence of the human immunoglobulin alpha 1 and alpha 2 constant region gene sequences. Cell 36: 681-688, 1984. PubMed: 6421489
Bensmana M, et al. Sequence of the CH1 and hinge-CH2 exons of the human immunoglobulin IGHA2 A2m(2) allele: comparison with the nonallelic and allelic IGHA genes. Cytogenet. Cell Genet. 56: 128, 1991. PubMed: 1901541
Marie-Paule Lefranc, personal communication
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