| 產品名稱 |
Suid herpesvirus 1 |
| 商品貨號 |
B188982 |
| Classification |
Herpesviridae, Varicellovirus |
| Deposited As |
Pseudorabies virus |
| Agent |
Suid herpesvirus 1 |
| Common Name |
Pseudorabies virus |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
derived from PRV dl tk (Kit), ATCC VR-2074 |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
Live marker vaccine of PRV attenuated by deletions in thymidine kinase gene. Also has deletion of PRV g92 (gIII) gene. Cultivate virus at an m.o.i. of 0.01 PFU/ml in rab cell culture at 34.5C. Harvest. Sonicate prior to infecting new cells. Virus replicates at 39C but does not induce thymidine kinase activity or a late glycoprotein due to deletion mutations. |
| Effect on Host |
Cytopathic effects in tissue culture |
| Recommended Host |
rabbit cells swine testicle cells; RAB-9 cells (ATCC ); cultures of bovine, murine, canine, feline or porcine cells |
| Growth Conditions |
Incubation:: 3 days at 30°C or 39°C |
| Name of Depositor |
NovaGene, Inc., Baylor College of Medicine |
| U.S. Patent |
|
| U.S. Patent Number |
|
| Source |
derived from PRV dl tk (Kit), ATCC VR-2074 |
| References |
Kit S, et al. Second-generation pseudorabies virus vaccine with deletions in thymidine kinase and glycoprotein genes. Am. J. Vet. Res. 48: 780-793, 1987. PubMed: 3035972
Kit M, Kit S. Pseudorabies virus mutants, vaccines containing same, methods for the production of same and methods for the use of same. US Patent 4,711,850 dated Dec 8 1987
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