| 產品名稱 |
Crypthecodinium cohnii (Seligo) Javornicky |
| 商品貨號 |
B189806 |
| Strain Designations |
MK8805 [MK8840, UTEX 1649] |
| Application |
Biofuel production |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Type Strain |
no |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Medium |
ATCC® Medium 2076: Crypthecodinium medium
|
| Growth Conditions |
Max Temperature: 30.0°C Min Temperature: 20.0°C Duration: axenic Protocol: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 2076 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen by one half turn, and incubate the culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times, aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2076, and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss. |
| Subcultivation |
Protocol: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 2076 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen by one half turn, and incubate the culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times, aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2076, and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss. |
| Name of Depositor |
Martek Biosciences Corporation, D Kyle, Martek Biosciences Corporation |
| U.S. Patent Number |
|
| U.S. Patent |
|
| Chain of Custody |
ATCC <<--Martek Biosciences Corporation, D Kyle, Martek Biosciences Corporation<<--UTEX 1649 |
| References |
Kyle DJ, et al. Infant formula and baby food containing docosahexaenoic acid obtained from dinoflagellates. US Patent 5,397,591 dated Mar 14 1995
Kyle DJ, et al. Production of docosahexaenoic acid by dinoflagellates. US Patent 5,407,957 dated Apr 18 1995
Kyle DJ, et al. Pharmaceutical composition and dietary supplement containing docosarexaenoic [docosahexaenoic] acid obtained from dinoflagellates. US Patent 5,492,938 dated Feb 20 1996
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