Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Unknown
Product Format
frozen
Storage Conditions
Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures: 2-8°C
Live Cultures: See Protocols section for handling information
Type Strain
no
Comments
Control strain for enterocytozoan identification
Growth Conditions
Temperature: 35°C
Cell Line: ATCC® CCL-26™ (kidney, African green monkey)
Cryopreservation
Harvest and Preservation
Harvest the culture by gently agitating the contents of each flask. Transfer all but approximately 1 mL of the culture medium to 15 mL plastic centrifuge tubes. Detach the remaining tissue culture cells (infected and uninfected) by scraping the surface of the flask with a cell scraper. Pass the resulting cell suspension through a syringe equipped with a 27 gauge 1/2 in needle and pool this suspension with the culture fluid.
Spin the cell suspensions at approximately 50 x g for 3 min, to remove the cellular debris.
Transfer the spore suspensions (supernatants) to new 15 mL plastic centrifuge tubes. Centrifuge at 1300 x g for 10 min.
Pool the spore pellets and adjust the concentration to 2.0 - 4.0 x 107 cells/mL with a fresh solution of Hank's Balanced Salt Solution.
*If the concentration is too low centrifuge at 1300 x g for 10 min and resuspend in the volume of Hank's Balanced Salt Solution required to yield the desired concentration.
Mix the spore preparation and 20% (v/v) DMSO in equal portions. The final concentration will be 1.0 - 2.0 x 107 cells/mL and 10% DMSO. The time from the mixing of the cell preparation and the cryoprotective solution before the freezing process begins should be no less than 15 min. and no more than 30 min.
Dispense in 0.5 mL aliquots to 1.0-2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
Store in either the vapor or liquid phase of a nitrogen refrigerator.
To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after thawed.
Immediately after thawing, aseptically transfer contents to a T-25 tissue culture flask containing a fresh monolayer of ATCC® CCL-26™ cells and 10 mL ATCC® 30-2003 with 3% (v/v) HIFBS.
Outgas the flask for 10 seconds with a 95% air, 5% CO2gas mixture.
Incubate in a 35°C CO2 incubator with the caps screwed on tightly.
Name of Depositor
GS Visvesvara
Special Collection
NCRR Contract
References
Croppo GP, et al. Identification of the microsporidian Encephalitozoon hellem using immunoglobulin G monoclonal antibodies. Arch. Pathol. Lab. Med. 122: 182-186, 1998. PubMed: 9499364
Xiao L, et al. Genotyping Encephalitozoon hellem isolates by analysis of the polar tube protein gene. J. Clin. Microbiol. 39: 2191-2196, 2001. PubMed: 11376056
Molestina R, Becnel JJ, Weiss LM. Culture and Propagation of Microsporidia. In Microsporidia: Pathogens of Opportunity, First Edition, Chapter 18: pp. 457-467, 2014. Hoboken, NJ: John Wiley & Sons, Inc.
